European Synchrotron Radiation Facility
BM29 - BioSAXS
The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Beamline Energy Resolution
1.5 * 10-2 [meV] @ 11 [eV]
Beamline Energy Range
7000 - 15000 [eV]
Max Flux On Sample
3 * 1013 [ph/s] @ 15 [eV]
Spot Size On Sample Hor
100 - 500 [um]
Spot Size On Sample Vert
100 - 500 [um]
Divergence Hor
130 [urad]
Divergence Vert
60 [urad]
Angle Of Incidence Light On Sample Value
90 [degrees]
Endstations or Setup
BioSAXS
Detectors Available
Pilatus 1M
Pilatus3 2M in-vac
Pilatus3 2M in-vac
Endstation Operative
Yes
Sample
Sample Type
Liquid
Mounting Type
in capillary
Required Sample Volume
30 [uL]
Detectors
Pilatus 1M
Type
1st geneation
Pixel Size
X = 172 [um], Y = 172 [um]
Array Size
X = 981 [pixel], Y = 1043 [pixel]
Passive or Active (Electronics)
Active
Dynamic Range
2 * 101 [bits]
Detection
Detected Particle
Photon
Pilatus3 2M in-vac
Type
3rd generation
Pixel Size
X = 172 [um], Y = 172 [um]
Array Size
X = 1475 [pixel], Y = 1679 [pixel]
Passive or Active (Electronics)
Active
Dynamic Range
20 [Bits]
Detection
Detected Particle
Photon
Support Laboratories
BM29 sample prep lab
Description
nearby the beamline, room 30-0-06. Available: cooled centrifuges, fridge, freezers (-20C and -80C), nanodrop spectrometers, ice machine, degaser, SEC pump
Techniques
Scattering
- Small angle scattering
Disciplines
Life Sciences & Biotech
- Medicine
- Molecular and cellular biology
- Other - Life Sciences & Biotech
control/Data analysis
Control Software Type
- SPEC, gui in framework 4, python
Data Output Type
- images, curves
Data Output Format
- edf (esrf data format), ascii
Softwares For Data Analysis
- edna, atsas
Equipment That Can Be Brought By The User
SEC column, microfluidics chips
