The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.

Beamline Energy Resolution
1.5 * 10-2 [meV] @ 11 [eV]
Beamline Energy Range
7000 - 15000 [eV]
Max Flux On Sample
3 * 1013 [ph/s] @ 15 [eV]
Spot Size On Sample Hor
100 - 500 [um]
Spot Size On Sample Vert
100 - 500 [um]
Divergence Hor
130 [urad]
Divergence Vert
60 [urad]
Angle Of Incidence Light On Sample Value
90 [degrees]
Monochromators

ESRF

Energy Range
7000 - 15000 [eV]
Type
double multilayer, diffracting in horizontal plane
Resolving Power
1.5 * 10-2 [deltaE/E] @ 15 [eV]
Number Of Gratings
120
Grating Type
[Ru/B4C], period 3nm
Refocusing Mirror Type
toroid
Endstations or Setup

BioSAXS

Detectors Available
Pilatus 1M
Pilatus3 2M in-vac
Endstation Operative
Yes

Sample

Sample Type
Liquid
Mounting Type
in capillary
Required Sample Volume
30 [uL]
Detectors

Pilatus 1M

Type
1st geneation
Pixel Size
X = 172 [um], Y = 172 [um]
Array Size
X = 981 [pixel], Y = 1043 [pixel]
Passive or Active (Electronics)
Active
Dynamic Range
2 * 101 [bits]

Detection

Detected Particle
Photon

Pilatus3 2M in-vac

Type
3rd generation
Pixel Size
X = 172 [um], Y = 172 [um]
Array Size
X = 1475 [pixel], Y = 1679 [pixel]
Passive or Active (Electronics)
Active
Dynamic Range
20 [Bits]

Detection

Detected Particle
Photon
Support Laboratories

BM29 sample prep lab

Description

nearby the beamline, room 30-0-06. Available: cooled centrifuges, fridge, freezers (-20C and -80C), nanodrop spectrometers, ice machine, degaser, SEC pump


contacts
Petra PERNOT
Mark Tully
Martha Brennich
David FLOT
Techniques
Scattering
  • Small angle scattering
Disciplines
Life Sciences & Biotech
  • Medicine
  • Molecular and cellular biology
  • Other - Life Sciences & Biotech
control/Data analysis
Control Software Type
  • SPEC, gui in framework 4, python
Data Output Type
  • images, curves
Data Output Format
  • edf (esrf data format), ascii
Softwares For Data Analysis
  • edna, atsas
Equipment That Can Be Brought By The User
SEC column, microfluidics chips
Possibility To Connect External Endstation
Yes
in partnership with